Part:BBa_K2814012
BxBI Integrase_ ssrA deg tag
BxbI Integrase triggers attP × attB recombination. The product of attP × attB recombination is an integrated prophage flanked by two new recombination sites, attL and attR, each containing half sites derived from attP and attB. In the absence of accessory factors the integrases mediate unidirectional recombination between attP and attB with greater than 80% efficiency. In the presence of a phage-encoded accessory protein, the recombination directionality factor (RDF) the attP × attB recombination is inhibited and the attL × attR recombination is stimulated. Bxb1 integrase yields approximately two-fold more recombinants and displays about two fold less damage to the recombination sites than other phage-encoded serine integrases.
Our BxbI Integrase has a ssrA deg tag attached to it for faster degradation rates.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 189
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 463
Illegal XhoI site found at 550 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1297
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